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Paper   IPM / Nano-Sciences / 15045
School of Nano Science
  Title:   Wide field of view common path lateral shearing digital holographic interference microscope
  Author(s): 
1.  Priyanka Vora
2.  Vismay Trivedi
3.  Swapnil Mahajan
4.  Nimit Patel
5.  Mugdha Joglekar
6.  Vani Chhaniwal
7.  Ali Reza Moradi
8.  Bahram Javidi
9.  Arun Anand
  Status:   Published
  Journal: J. Biomed. Opt.
  No.:  12
  Vol.:  22
  Year:  2017
  Pages:   126001
  Publisher(s):   Society of Photo-Optical Instrumentation Engineers
  Supported by:  IPM
  Abstract:
Quantitative three dimensional imaging of living cells provide important information about the cell morphology and its time variation. Off-axis, digital holographic interference microscopy is an ideal tool for 3D imaging, parameter extraction and classification of living cells. Two-beam digital holographic microscopes, which are usually employed, provides high quality 3D images of micro-objects, albeit with lower temporal stability. Common-path digital holographic geometries, in which the reference beam is derived from the object beam provides higher temporal stability along with, high quality 3D images. Selfreferencing geometry is the simplest of the common-path technique, in which a portion of the object beam itself act as the reference, leading to compact setups,using few optical elements. But it has reduced field of view and also the reference may contain object information. Here we describe the development of a common-path digital holographic microscope, employing a shearing plate and converting one of the beams into a separate reference by employing a pin-hole. The setup is as compact as self referencing geometry, while providing field of view as wide as that of a two-beam microscope. The microscope is tested by imaging and quantifying the morphology and dynamics of human erythrocytes.

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