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In general relativistic framework of the large scale structure formation theory in the universe, we investigate the relation between density contrast and peculiar velocity in the Newtonian gauge. According to the gauge-invariant property of the energy-momentum tensor in the Newtonian gauge, we consider the perturbation of velocity in the energy-momentum tensor behaves as the Newtonian peculiar velocity. It is shown that in the relativistic framework, the relation between peculiar velocity and density contrast has an extra correction term with respect to the Newtonian Peebles formula which in small scales, can be ignorable . The relativistic correction of peculiar velocity for the structures with the extension of few hundred mega parsec is about few percent which is smaller than the accuracy of the recent observations for measuring peculiar velocity. The peculiar velocity in the general relativistic framework also changes the contribution of Doppler effect on the anisotropy of CMB.
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