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Paper   IPM / Biological / 13294
School of Biological Sciences
  Title:   Design and characterization a chimeric multi-epitope construct containing CfaB, ST toxoid, CssA, CssB and LTB of Enterotoxigenic
  Author(s): 
1.  N. Zeinalzadeh.
2.  G. Ahangari.
3.  M. Sadeghi.
4.  J. Amani.
5.  S. Z. Bathaie.
6.  M. Jafari.
  Status:   Published
  Journal: Biotechnology and applied biochemistry
  Year:  2013
  Supported by:  IPM
  Abstract:
Enterotoxigenic Escherichia coli (ETEC) strains are the most common cause of bacterial diarrhea in children in developing countries and travelers to these areas. Enterotoxins and colonization factors (CFs) are two key virulence factors in ETEC pathogenesis, and the heterogeneity of the CFs is the bottleneck in reaching an effective vaccine. In this study, a candidate subunit vaccine, which is composed of CfaB, CssA and CssB, structural subunits of colonization factor antigen I and CS6 CFs, labile toxin subunit B, and the binding subunit of heat-labile and heat-stable toxoid, was designed to provide broad-spectrum protection against ETEC. The different features of chimeric gene, its mRNA stability, and chimeric protein properties were analyzed by using bioinformatic tools. The optimized chimeric gene was chemically synthesized and expressed successfully in a prokaryotic host. The purified protein was used for assessment of bioinformatic data by experimental methods. doi: 10.1002/bab.1196

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